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TUNEL assays were executed on paraffin sections of WT and mutant hearts with an In Situ Cell Death Detection Kit (Roche, 11684817910) that labeled the nuclei of dying cells with green fluorescence. Cardiomyocytes were co-stained by immunostaining with cTnT antibody (1:500, clone 1C11, Abcam, ab8295) and Alexa Fluor 555 goat anti-mouse IgG (1:1000, Thermo Fisher, A21422).
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