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Functional enrichment analysis and immune-related analysis of differential genes in the two risk subgroups

JZ Junde Zhao
JZ Jiani Zou
WJ Wenjian Jiao
LL Lidong Lin
JW Jiuling Wang
ZL Zhiheng Lin
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Firstly, the “limma” R package was used to analyze the differential expression of genes between the high-risk and low-risk groups. Genes with |log2 FC|> 0.1 and FDR < 0.05 were differentially expressed between the high-risk and low-risk groups. The next step was to do the GO analysis. The infiltration scores of 16 immune cells and 13 immune functions were calculated for ssGSEA using the “GSVA” R package in UCEC. The activities of immune checkpoints and m6A RNA methylation regulatory factors were analyzed in the high-risk and low-risk groups. P < 0.05 was considered significant in all statistical analysis.

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