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RNA Isolation and Real-Time qPCR.

CR Cristian V. Riella
MM Michelle McNulty
GR Guilherme T. Ribas
CT Calum F. Tattersfield
CP Chandra Perez-Gill
FE Felix Eichinger
JK Jessica Kelly
JC Justin Chun
BS Balajikarthick Subramanian
DG Dieval Guizelini
SA Seth L. Alper
MP Martin R. Pollak
MS Matthew G. Sampson
DF David J. Friedman
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RNA was isolated per the Qiagen RNeasy Kit protocol (Qiagen). Complementary DNA synthesis was performed with SuperScript IV VILO (Invitrogen by Thermo Fisher Scientific). Real-time qPCR was performed with the QuantStudio 6 Flex platform and the TaqMan Universal PCR Master Mix (Applied Biosystems by Thermo Fisher Scientific). The TaqMan gene expression assay for β-actin mRNA was used for normalization. Relative expression was calculated via the 2–ΔΔCT method.

Copyright and License information:  ©2022 the Author(s). Published by PNAS.
This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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