Determination of malondialdehyde (MDA), GSH and GSSH level

PZ Pengzhan Zhao
YW Yutian Wei
GS Guangchi Sun
LX Lei Xu
TW Tian Wang
YT Yufei Tian
HC Honglu Chao
YT Yiming Tu
JJ Jing Ji
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The MDA, GSH and GSSH level was measured with the Lipid Peroxidation MDA Assay Kit (S0131S, Beyotime Biotech, China) and GSH and GSSG Assay Kit (S0053, Beyotime Biotech, China). After the different treatment described above, the cells were washed with PBS (pH 7.4) and were lysed subsequently. The lysates were centrifuged at 12,000 rpm for 10 min at 4 ℃. Then, the supernatant was collected and the absorbance at 532 nm was measured by a microplate reader (Biotech, Winooski, VT, USA). Finally, the datum was normalized by the protein concentration in each sample.

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