Mitochondrial and ER content

PK Prasanna Katti
PA Peter T. Ajayi
AA Angel Aponte
CB Christopher K. E. Bleck
BG Brian Glancy
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Light microscopic images were opened using ImageJ and binarized and the total mitochondrial volume was determined as the percentage of binarized mitochondrial pixels per total muscle fiber pixels. ER content was calculated as the percentage of binarized ER pixels per total muscle fiber pixels

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