2.1. Experimental materials

SN-38 and VER were purchased from Shanghai Aladdin Biochemical Technology Co., LTD. CCK-8 was purchased from Tongren Institute of Chemistry (Japan). RNA extraction and reverse transcription kits were purchased from TIANGEN Company. Antihuman rat MACC1 and D-glyceraldehyde-3-phosphate dehydrogenase antibodies were purchased from Sigma Company. Antirat sheep HRP-marked second antibodies were purchased from Guizhou Jinqiao Biological Company. High-throughput sequencing was authorized by Guangzhou Ruibo Company. siRNA used in gene transfection was bought from Guangzhou Ruibo Company. The overexpression plasmid and empty carrier was TrueORF GOLD (ORIGENE). The annexin V-PI dual-staining kit was bought from Beijing Beibo Company. Primer design and synthesis kits were bought from Shanghai ShineGene Molecular Biotechnology Co., LTD. Human colon cancer cells SW480, HT29 and HCT116 were purchased from the cell library of Chinese Academy of Sciences.