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NextSeq basecall files were converted to FASTQ files using bcl2fastq (v.2.17.1.14). Reads were screened and preprocessed similarly to the described above, an additional step of removing polyA sequences from the 3′ ends of reads was performed with cutadapt, using a 75-mer oligo-A sequence as the “adapter” and a minimal overlap of 2.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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