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Dorsal root ganglion (DRG) culture and whole-cell electrophysiology
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DRG culture and whole cell recordings were performed as in Park et al.28. Briefly, DRG neurons were dissociated from mice 7 days after DiI (ThermoFisher) injection. Neurons were recorded and optically stimulated with an EPC10 amplifier (HEKA Instruments) and Patchmaster software (HEKA Instruments). Optical stimulation was delivered through the microscope objective, using a custom set-up with a green (530 nm) LED (M530L3; Thorlabs). Light intensity of the LED at the focal plane was 10 mW/mm2. For more detailed methods see supplementary methods.
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