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2.4. Internals standards and precipitation reagents
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Stock internal standard solutions of MPA-d3 (1 mg/mL), MPAG-d3 (1 mg/mL) and AcMPAG-d3 (1 mg/mL) were prepared in methanol and stored at −70 °C. The protein precipitation reagent was 100% methanol and contained MPA-d3, MPAG-d3, and AcMPAG-d3 at 50 ng/mL, 1,000 ng/mL, and 100 ng/mL, respectively.
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