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Acridine orange staining was used to detect acidic vacuoles, as previously described [24]. Acridine orange emits red fluorescence in acidic environments, including lysosomes and autolysosomes. The fluorescence intensity of acridine orange was used to assess the formation of autophagolysosome.
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This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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