2.10. RT-PCR

The total RNA of D1-like, D2-like, CaM, and CaMKII from the striatum or hippocampus samples was extracted using Trizol reagent according to the manufacturer's instructions. RNA was reverse transcribed to cDNA using a TIANScript RT Kit. cDNA was used as a template to perform PCR amplification with an SYBR FAST qPCR Kit Master Mix (2×) Universal (US KAPA Biosystems). The specific primers are shown in Table 2.

Specific gene primers.

Each 20 μL reaction consisted of 2 μL of cDNA, 10 μL of SYBR Master Mix (2×) Universal, and 10 μmol/L of each primer (sense and antisense primers). Three replicates of each PCR run were performed. The mRNA concentrations of all target genes were normalized to that of GAPDH in each sample (using the delta-delta-Ct method).