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To assess the composition of immune cells in the tumor microenvironment, we used the ESTIMATE algorithm to evaluate the immune score, tumor purity, and stromal score of tumor samples. The relative abundance of 24 tumor-infiltrating immune cells was obtained by single sample gene set enrichment analysis (ssGSEA) through the R package “GSVA.” We then obtained gene sets of exhausted CD8+T cells, naive NKT cells, and nonactivated NK cells from the Molecular Signatures Database (MSigDB) and calculated their enrichment using the GSEA algorithm.
Copyright and License information: ©2022 Yang, Zhang, Shang, Chen, Mu, Zhang, Zheng, Wang and Liu.
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