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Slides of the suspension stained with 1% eosin Y were prepared and dried in room air. Then the slides were fixed with 96% ethanol and stained with the Papanicolaou method. The slides were viewed on a light microscope at 1000x magnification and the proportions of sperm with normal morphology were calculated. Sperm with unstructured or dual heads, combined bodies and dual, segmented or angled tails were considered abnormal (Momeni & Daneshpajoh, 2012).
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