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hTERT-PrEC cells were stably transduced with the pHAGE (empty vector control) and pHAGE-ALK16–29. After selection in puromycin, cells were seeded at a density of 7,000 cells/well in 96-well plates. DMSO or crizotinib (Selleckchem) was added 24 hours postseeding in triplicate wells. Cell proliferation was monitored using a Cytation5 live cell imaging instrument (BioTek). Images were acquired every 12 hours and image analysis was performed using the Gen5 software (BioTek).
Copyright and License information: ©2022 The Authors; Published by the American Association for Cancer Research
This open access article is distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license.
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