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Monocyte isolation and culture

YC Yue Cui
SG Saray Gutierrez
SA Sheller Ariai
LÖ Lisa Öberg
KT Kristofer Thörn
UG Ulf Gehrmann
SC Suzanne M. Cloonan
TN Thomas Naessens
HO Henric Olsson
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Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by density centrifugation using Lymphoprep (STEMCELL Technologies) or Ficoll-Paque (GE Healthcare). Monocytes were isolated from PBMCs using Classical Monocyte Isolation Kit (Miltenyi Biotec) or Monocyte Isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions. Monocytes were differentiated for 3-5 days in X-Vivo 15 media (Lonza) supplemented with 4mM L-glutamine, 100 U/mL penicillin/streptomycin (Thermo Fisher Scientific), and 100 ng/mL M-CSF (PeproTech) to generate monocyte-derived macrophages (MDMs).

Copyright and License information:  ©2022 Cui, Gutierrez, Ariai, Öberg, Thörn, Gehrmann, Cloonan, Naessens and Olsson
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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