i) Hole-board test

Studying the exploratory activities of mice, this test is widely recognized as a way to assay potential sedative and anxiolytic effects of any compound. The equipment contained 16 equal size and evenly spaced holes. Normal saline (0.1 mL/mice, p.o.) and diazepam (1 mg/kg, i.p.) were given to the control and standard groups, respectively. It was established that the animals’ head-dipping activity is directly relevant to their emotional situation.¹⁹ The mice were put on the perforated flat platform after a period of 30 min in the case of the control and crude extract, and 15 min in the case of the standard, and the head dips’ number in a period of five minutes was registered.