Aβ1-42-Induced AD Mice Model

Aβ_1-42 dissolved in 0.89% NaCl solution was incubated at 37°C for 3 days for Aβ aggregation. Then, Aβ was injected to a depth of 2.5 mm in bregma with a Hamilton micro-syringe fitted with a 26-gauge needle (10 μl, intracerebroventricular (i.c.v.) injected). The NC and NS groups were injected with 10 μl of 0.89% NaCl without Aβ solution, and the Aβ, P15 and P30 groups were injected with 10 μl of 0.89% NaCl, including 410 pM Aβ solution [17]. A behavioral experiment was performed 3 days after Aβ injection to allow the experimental animals to recover from surgical treatment.