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Cytometric Bead Assay

LC Liliana Czernek
ŁP Łukasz Pęczek
MD Markus Düchler
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The PBMC-conditioned media were tested for the presence of soluble cytokines. Samples were measured using Cytometric Bead Arrays (BD Biosciences, USA) for IL-10 (Cat# 558,274, BD) and TGF-β (Cat# 560,429, BD) according to the manufacturer protocols. All samples were analysed using a BD FACS Calibur flow cytometer (BD Bioscience, USA) and the FCAP Array software (BD version 3.0). A ten-point standard curve was included.

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