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Determination of minimum inhibitory concentration (MIC)
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Determination of minimal inhibitory concentration (MIC) for Alhagi graecorum ethanolic extract, sodium benzoate, and sodium propionate was performed using the microbroth dilution method according to Andrews (2001). Twofold serial dilutions of the ethanolic extract, sodium benzoate, and sodium propionate ranging from 5 to 0.1 mg mL−1 were used. Equal volumes of tested bacteria (105 CFU/mL) were added to each well. The MIC was defined as the lowest concentration of antimicrobial agent that was able to inhibit the bacterial growth after 24 h of incubation at 37 °C.
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