The Bristol strain (N2) was used as the wild-type strain. SJ4100 [zcIs13(hsp-6p::GFP)], MQ887 [isp-1(qm150)IV], MQ130 [clk-1(qm30) III], SJ4005 [zcIs4(hsp-4p::GFP)], CL2070 [dvIs70 (hsp-16.2p::GFP)], SJ4197 (zcIs39[dve-1p::dve-1::GFP]), OP506 (wgIs506 [xbp-1::TY1::EGFP::3xFLAG + unc-119(+)]). OP675 (wgIs675 [atfs-1::TY1::EGFP::3xFLAG + unc-119(+)]), VC222 [raga-1(ok386)], HZ1683 [atg-2(bp576)], HZ1684 [atg-3(bp412)], HZ1686 [atg-7(bp411)], HZ1687 [atg-9(bp564)], RW12006 (stIs12006 [atfs-1::H1-wCherry + unc-119(+)]), RB967 [gcn-2(ok871)], and RB545 [pek-1(ok275)] were obtained from the Caenorhabditis Genetics Center (CGC). Worms were routinely maintained at 20°C and fed with Escherichia coli (E. coli) OP50 (RRID:WB-STRAIN:WBStrain00041971) on Nematode Growth Medium (NGM) plates.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.