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Acute itch behavior

JF Jing Feng
PY Pu Yang
MM Madison R. Mack
DD Dariia Dryn
JL Jialie Luo
XG Xuan Gong
SL Shenbin Liu
LO Landon K. Oetjen
AZ Alexander V. Zholos
ZM Zhinan Mei
SY Shijin Yin
BK Brian S. Kim
HH Hongzhen Hu
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For the intradermal injections of SADBE, a 2.3 M stock solution was made by dissolving SADBE in DMSO with a ratio of 1:1 (volume). The final 30 mM SADBE solution was prepared by 1:77 dilutions in 0.9% saline. Mice were shaved on the nape of the neck or in the face 2 days before assay. On the day of experiment, mice were acclimated for 1 h by placing each of them individually in the recording chamber followed by intradermal injection of SADBE to the nape of the neck and/or cheek. Immediately after the injection, mice were videotaped for 30 min without any person in the recording room. After the recording, the videotapes were played back and the number of scratching bouts towards the injection site was counted by an investigator blinded to the treatment.

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