High fat feeding

Adipo-Gatm KO mice and age-matched littermate controls (Gatm flox/flox and Adipo-Gatm heterozygous) were genotyped at 2 weeks of age. At three weeks of age, mice were weaned by genotype into groups of three per cage, where they were housed at 23°C and fed a chow diet. Mice of different genotypes were housed in cages side by side to limit variability in exposure to differential temperatures within the housing facility. At 8 weeks of age, mice (housed three per cage) were transferred to incubators set at 28°C and directly switched to high fat diet, which was a rodent diet (OpenSource Diets, D12492) with 60% kcal% fat, 20% kcal% carbohydrate, and 20% kcal% protein. C57BL/6NJ mice (Jackson labs; stock No: 005304) were used as wild type mice for examining mRNA levels during diet-induced thermogenesis studies.