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2.2. Animal Preparation

DK Datu Agasi Mohd Kamal
SI Siti Fatimah Ibrahim
AU Azizah Ugusman
SZ Siti Sarah Mohamad Zaid
MM Mohd Helmy Mokhtar
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All experimental procedures were reviewed and approved by the National University of Malaysia Animal Ethics Committee with the ethical approval code FISIO/FP/2020/MOHD HELMY/14-MAY/1104-JUNE-2020-MAY-2023. In this study, female Sprague-Dawley (SD) rats with body weights of between 120 and 150 g and with at least two continuous normal oestrus cycles were used. All experimental rats were received from the Laboratory Animal Research Unit (LARU), Faculty of Medicine, Universiti Kebangsaan Malaysia. All rats were housed under standard housing conditions (room temperature 24 ± 2 °C, 12 h light and 12 h dark cycle, one rat per cage) and were acclimatized for a week. All rats were fed a normal rat diet and provided with water ad libitum.

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