Cell lines and cell culture

JL Jiayan Luo
JJ Juan Jin
FY Fang Yang
ZS Zijia Sun
WZ Wenwen Zhang
YS Yaqin Shi
JX Jing Xu
XG Xiaoxiang Guan
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TNBC cell lines MDA-MB-231 and HCC1937 were obtained from the American Type Culture Collection (Manassas, VA, USA). Both of them were cultured in RPMI 1640 medium (GIBCO, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Cell cultures were maintained at 37 °C with 5% CO2 in a humidified atmosphere.

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