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2.20. Dual Luciferase Reporter Test
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The plasmids (labeled with firefly luciferase) containing wild-type or mutant fragments of EIF5A2 3′-UTR and miR-1275 mimic or NC duplex (labeled with Renilla luciferase) were co-transfected to HEK293T cells for 2 days. Then HEK293T cells were lysed, and the luciferase activity was measured under the guidance of the instructions of the Bio-Lite Luciferase Assay System (Vazyme, Nanjing, China) by a fluorometer.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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