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GlycatedPE (100 µg) was loaded in sample buffer (1 mL, mixture of methanol and ammonium hydroxide, 95:5, v/v) on a m-aminophenylboronic acid–agarose column (1 mL propylene column; Qiagen GmbH) [25] and washed with sample buffer (2 mL). Glycated lipids were eluted with methanol (5 mL) and dried under a stream of nitrogen.
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