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Lungs were excised, fixed in 10% neutral buffered formalin (pH 7.4) for 48 h, cleaved, embedded in paraffin, 4 μm-thick sections were obtained, stained with hematoxylin and eosin (H&E), and examined under light microscopy. Neoplastic cells form aggregates, or tumor nodules were considered metastatic lung lesions. The total lung area and area of metastatic lung lesion were measured using images captured at Olympus BX41 microscope in the SPOT 3.4.5 software.
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