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HUVECs were cultured on the culture inserts of u-dishes (ibidi, 81156) until 80% confluence. The culture inserts were subsequently removed to generate wound gaps. HUVECs were then incubated in EGM-2 (Promocell, C-22010) without SupplementMix (Promocell, C-39215) containing 5 nM VEGFA and 25 nM of VEGF-Grab or Ate-Grab for 24 h. Migrated cells within the wound were determined.
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