Ultrasound imaging was performed to measure tumor size twice or thrice a week using the Vevo L3100 system (VisualSonic) with an MX550D probe (frequency: 40 MHz). Tumor-bearing mice were anesthetized via intraperitoneal injection of ketamine-xylazine solution. After shaving, the exposed skin was covered with ultrasound gel, and images were acquired upon applying the probe. Tumor mass in the abdominal cavity was defined as low echogenicity from the normal pancreas. The long diameter (LD) and short diameter (SD) were measured. Tumor volume was as: tumor volume = (LD × SD2)/2. All drugs were administered intraperitoneally every 3 or 3.5 days. According to IACUC, tumor size must not exceed 20 mm (2.0 cm) at the largest diameter in mice, and the maximal tumor size in this study was not exceeded.
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