We wished to assess the level of homology between the linear sequences of the VL antigens and potential cross-reacting species. Thus, the HASPB1, HASPB2, and kinesin amino acid sequences of rK28 (GenBank {"type":"entrez-nucleotide","attrs":{"text":"HM594686","term_id":"313747924"}}HM594686) and the 46-amino acid non-repeat region and 6.5 × 39-amino acid repeats of rK39 (GenBank {"type":"entrez-nucleotide","attrs":{"text":"L07879","term_id":"308884"}}L07879) were submitted to BLASTp searches (https://blast.ncbi.nlm.nih.gov), both unrestricted by taxon and restricted to Schistosoma, Plasmodium, and Mycobacterium.
We used BepiPred-2.0 (https://services.healthtech.dtu.dk/service.php?BepiPred-2.0) to predict linear B-cell epitopes in the HASPB1 sequence of rK28 (GenBank {"type":"entrez-nucleotide","attrs":{"text":"HM594686","term_id":"313747924"}}HM594686).
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