We used FMDV isolates of O/TAI/315/2016, belonging to serotype O, topotype ME-SA and linage Ind-2001e, and A/MOG/2013, belonging to serotype A, topotype ASIA and linage Sea-97. O/TAI/315/2016 isolate was obtained from a tongue epithelial sample from cattle in Songkhla province in Thailand in November 2016. The virus was initially isolated from primary lamb kidney cells and subsequently passaged twice in ZZ-R 127 and IB-RS-2 cells [27, 28]. Furthermore, the virus was additionally passaged once in ZZ-R 127 cells before use for experimental infections. A/MOG/2013 isolate was kindly supplied by the Pirbright Institute, UK, and passaged once in bovine thyroid cells, twice in BHK-21 cells [29] and once in IB-RS-2 cells before experimental infections.
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