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HR Hannah Drew Rickner
LJ Lulu Jiang
RH Rui Hong
NO Nicholas K. O’Neill
CM Chromewell A. Mojica
BS Benjamin J. Snyder
LZ Lushuang Zhang
DS Dipan Shaw
MM Maria Medalla
BW Benjamin Wolozin
CC Christine S. Cheng
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Images were captured by Carl Zeiss confocal LSM700 and confocal Zeiss LSM 880 with Airyscan. The immunofluorescence-stained DAPI-positive cells in each image of asteroids were quantified by ImageJ by automated cell counting. The staining intensity in immunofluorescence-labeled asteroids were measured by ImageJ. The intensity of MC1, TOMA2, CP13, AT8, ThioS, and Fluoro Jade B were normalized by the corresponding Tuj1 intensity. Schematics were created with BioRender.com.

Statistical analyses and figures artwork were performed using GraphPad Prism version 9.00 for Windows with a two-sided α of 0.05. All group data are expressed as mean ± SEM. Column means were compared using one-way ANOVA with treatment as the independent variable. Group means were compared using two-way ANOVA with factors on oTau treatment and timepoints, respectively. When ANOVA showed a significant difference, pairwise comparisons between group means were examined by Tukey’s, Dunnett or uncorrected Fisher’s LSD multiple-comparison test. Significance was defined when P < 0.05. LDH assay data analysis was performed with a paired t test.

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