Statistical analyses and figures artwork were performed using GraphPad Prism version 9.00 for Windows with a two-sided α of 0.05. All group data are expressed as mean ± SEM. Column means were compared using one-way ANOVA with treatment as the independent variable. Group means were compared using two-way ANOVA with factors on oTau treatment and timepoints, respectively. When ANOVA showed a significant difference, pairwise comparisons between group means were examined by Tukey’s, Dunnett or uncorrected Fisher’s LSD multiple-comparison test. Significance was defined when P < 0.05. LDH assay data analysis was performed with a paired t test.
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