Plasmid, miRNA mimic and inhibitor transfection

FF Fang Fang
LX Lei Xu
LL Liqing Liang
MQ Mingyi Qu
HY Hailei Yao
WY Wen Yue
LC Lin Chen
DC Dongli Chen
ZF Zeng Fan
LH Lijuan He
XN Xue Nan
HZ Huanhuan Zhang
XX Xiaoyan Xie
XP Xuetao Pei
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Pri-miR-125b2 coding sequence was transfected into K562 cells through a pcDNA3.1-neomycin vector. Stable transfections were selected with 500 μg mL−1 G418. Bcl-2 coding sequence (pCEP4 Bcl-2, #16461, Addgene, Cambridge, MA, USA) was transfected and selected with 50 μg mL−1 hygromycin B.

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