Labeling for EdU:

Regular drinking water was removed 24hr prior to NMDA injections and replaced with water containing 5-ethynyl-2′-deoxyuridine (EdU; Sigma; 50 mg/100 mL dH2O) and EdU water was replaced every third day. Mice were maintained on EdU water until 4^th day after TSA treatment.

Immunolabeled tissue sections were fixed in 4% formaldehyde in PBS for 5 minutes at room temperature, washed for 10 minutes with PBS, permeabilized with 0.01% Triton X-100 in PBS for 1 minute at room temperature, and washed in PBS for 10 minutes. Sections were incubated for 30 minutes at room temperature in 2M Tris, 50 mM CuSO₄, Alexa Fluor 568 or 647 Azide (Thermo Fisher Scientific), and 0.5M ascorbic acid in dH₂O. Sections were washed with PBS and further processed for immunofluorescence as required.