2.7. Quantitative Reverse Transcription-PCR (qRT-PCR)

Total RNA samples were extracted using TRIzol reagent (Invitrogen, USA). The RNAs were then reverse transcribed into cDNA using PrimeScript RT Master Mix kit (RR036A; Takara) in accordance with the descriptions. Next, the PCRs detection was performed using 2 × SYBR Green PCR Mastermix (Solarbio, Beijing, China) in a 7500 Real-Time PCR System (Applied Biosystems, USA). Primers applied are shown in Table 1.

Primer sequences.