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Generation of protein expression vectors.
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A gene fragment encoding Escherichia coli-codon-optimized OprF190-342 and OprI21-83 was synthesized with a 5 prime ndeI and a 3 prime xhoI restriction site (Genescript). This fragment was then cloned into the ndeI and xhoI site of pET24a(+) creating pET24a(+)-OPRF/I. Insert was confirmed by Sanger sequencing. The pET28b-based PopB/PcrH expressing vector was previously described (23).
Copyright and License information: ©2022 Shaikh et al.
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