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2.3 Insulin-like growth factor 1 plasma levels
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IGF-1 plasma levels were determined in 10 fish per group at the middle (4 weeks) and end (8 weeks) of the experimental trial after sample extraction by the acid-ethanol method (Shimizu et al., 2000). The concentration of IGF-1 was measured using the Fish IGF-1 ELISA kit (CSB-E12122Fh, CUSABIO, Wuhan, China), according to the manufacturer’s instructions, as previously done with plasma samples of the same species by Rodríguez-Viera et al. (2022).
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