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Protein extraction for MALDI-TOF analysis
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Mosquito proteins were extracted using legs, according to the protocol previously described [29]. To create the reference main spectrum pattern (MSP), eight spots of the same sample were deposited on the plate. Conversely, each sample intended to be queried against these reference spectra were deposited in duplicate, as suggested by previous work [29]. To control matrix quality (i.e. absence of MS peaks due to matrix buffer impurities), the matrix solution was loaded in duplicate onto each MALDI-TOF plate.
Copyright and License information: ©2022 Rakotonirina et al
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