2.2. ERG

Longitudinal electroretinographic recordings were carried out in untreated (n = 5) and taurine treated (n = 5) animals at P24 and P45, following previously described methods [56,57]. Briefly, in dark-adapted animals, retinal responses were recorded simultaneously from both eyes with bipolar Brian-Allen electrodes placed on the corneas. A reference electrode was placed inside the mouth and a 30G needle placed subcutaneously at the base of the tail as a ground electrode. Electrical signals were digitized at 20khZ using a Power Lab digitizer card (AD Instruments, Chalgrove, UK). In scotopic conditions, rod response and mixed response were recorded with a light intensity of −2,5 log cd·s/m2 and 0.5 log cd·s/m2, respectively. After adapting the animals to light for 5 min (30 cd/m2), we registered the photopic b-wave was recorded with a light intensity of 0.5 log cd·s/m2. The stimulation and recording protocols were done following the recommendations of the International Society for Clinical Electrophysiology of Vision (ISCEV).