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Preparation of plant material and root nodulation
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B. diazoefficiens USDA 110 cultures were grown in YEMA broth (Vincent 1970) at 25°C for 48 h on a rotary shaker at 140 rpm till it reached stationary phase. Glycine max seeds were surface sterilised, germinated and then infected with B. diazoefficiens USDA 110 suspension for 2 h. For control, the same protocol was followed with plain YEMA broth. After infection, seedlings were planted in sterile vermiculate bags and kept in the green house. Matured nodules were harvested on 28th, 29th and 30th days. RNA isolation was performed immediately.
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