Cell Culture

T24 cells (ATCC, catalog no. HTB-4, RRID:CVCL_0554) were cultured in McCoy's medium, UM-UC-3 (ATCC, catalog no. CRL-1749, RRID:CVCL_1783) in DMEM, TCC-SUP (ATCC, catalog no. HTB-5, RRID:CVCL_1738) in MEM plus 1x MEM non-essential amino acid, HB-CLS-1 (CLS, catalog no. 300190/p466_HB-CLS-1, RRID:CVCL_6213) in RPMI1640, BO2 in enriched F-12K medium supplemented with ROCK inhibitor Y-27632 (10 μmol/L) and insulin growth factor (5 μg/mL) as reported previously (29), SV-HUC (ATCC, catalog no. CRL-9520, RRID:CVCL_3798) in F12-K medium. Media for all lines was supplemented with 10% FBS and penicillin/streptomycin. All media purchased from Corning. All cell lines were maintained at 5% CO₂, 37°C. Cells were tested for Mycoplasma via MycoAlert Mycoplasma Detection Kit (Lonza, catalog no. LT07-218) after thawing and minimally once every 3 months thereafter. All experiments were performed using cell lines passage 20 or below.