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Evaluation of insulin-stimulated signal transduction.
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Mice were anesthetized with isoflurane after a 6-h fast starting at 7 a.m. and were injected with 0.001 U insulin or saline via the inferior vena cava. Hearts were harvested 5 min post-injection, snap-frozen in liquid nitrogen, and processed for immunoblot analysis.
Copyright and License information: ©2022 Riehle et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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