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Assessment of lymphocyte infiltration and CD8 staining
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The density of the lymphocyte infiltration in tumors was assessed by pathologist. A condition that is characterized by prominent lymphocytic infiltration was defined as high-lymphocyte infiltration; otherwise it was defined as low-lymphocyte infiltration (2, 18). The staining was performed according to the manufacturer’s protocol with the CD8 marker (ZSGB-BIO, ZA-0508). In brief, the sections were incubated with primary antibodies to CD8 (1:100) overnight at 4°C and then analyzed with streptavidin peroxidase detection system (Maixin Technology Co., Ltd., China).
Copyright and License information: ©2022 He, Han, Xu, Huang, Li, He and Lin.
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