The procedure for detecting lymphocyte subsets

According to the sample preparation instructions provided by the manufacturer, two flows of sample tubes should be taken, and they should be sequentially numbered with the test number; The procedure for using quality control materials (Beckman Coulte, Inc., Brea, CA, USA) is the same as the procedure for using standard sample tests. First, 10 μl of CD3/CD8/CD45/CD4 and CD3/CD16⁺CD56/CD45/CD19 antibodies (Mindray, Shenzhen) were injected into the numbered flow sampling tubes, respectively. After inverting and mixing the blood samples 6–8 times, 50 μl of blood samples were injected into the corresponding numbered tubes by the trans-addition technique, mixed well, and left at room temperature in the dark for 15 min. Then, 450 μl of red blood cell lysates (1:9 dilution with deionized water) were injected into each tube, mixed well, and left again at room temperature in the dark for 15 min. Finally, the tests were performed on a calibrated instrument.