ChIP–qPCR Assay

ChIP experiments were performed on mouse primary hepatocytes using a SimpleChIP Enzymatic Chromatin IP Kit (9003; Cell Signaling) according to the manufacturer’s procedures, with additional antibodies against ALKBH1 (ab195376; Abcam) and N6-methyladenosine (202 003; Synaptic Systems). Cells were fixed with formaldehyde to covalently cross-link protein to DNA to conserve their interactions. After immunoprecipitation, pulled-down DNA and the input DNA were subjected to qPCR analysis with the primers (Table 2) for the indicated genomic sequence. The results are presented as the percentage of input DNA.

List of Primer Sequences for ChIP-qPCR