Dual‐luciferase reporter assay

The wild‐type and mutant‐type of circ_0002476 or TFAM 3′UTR vectors were constructed by cloning these sequences into pmirGLO reporter vector. The WT/MUT‐circ_0002476 or WT/MUT‐TFAM 3′UTR vectors were transfected into NSCLC cells with miR‐1182 mimic/miR‐NC. Forty‐eight hours, Dual‐Luciferase Reporter Assay Kit (Vazyme) was used to measure relative luciferase activity (Firefly/Renilla).