2.4. Measurement of CK, LDH, GSH and MDA Levels

Serum and cardiac tissues were acquired by every group of mice and stored at −80 °C for subsequent analyses. To analyze the cardiac injury, the levels of creatine phosphokinase (CK) and lactate dehydrogenase (LDH) in serum were detected by using a colorimetry kit (Nanjing Jiancheng Biological Engineering Institution, Nanjing, China) and a commercial kit (Solarbio, Beijing, China). To explore the oxidative stress levels, the content of glutathione (GSH) in serum and the malondialdehyde (MDA) levels in cardiac tissues were tested by using the commercial assay kits (Solarbio) according to the instructions, respectively.