3.9.2. Salmonella typhimurium

A promising approach for the preparation of OS involves bacteriophages, which can produce enzymes that are able to degrade PSs in order to gain access to the bacterial cell surface [92]. Enzymatic degradation with phage 36 endo-rhamnosidase of partially delipidated S. Typhimurium O-SP (Figure 1D) was used for the production of OS with 4, 8 or 12 RU, which were then conjugated through the terminal reducing ends to BSA, and the immunogenicity induced by the corresponding conjugate vaccines was compared in mice [43].