4.11. Measurement of SOD-3 Expression in the Nematodes

The young adult CF1553 worms were transferred onto NGM agar plates with or without ellagic acid for two days and exposed to UV radiation of 254 nm at a dose of 1000 J/cm². After being transferred onto new NGM agar plates for 12 h, the CF1553 worms were anesthetized with 50 mM sodium azide and put onto a glass slide covered with 5% agar. Photos were taken using a fluorescence microscope and the fluorescence intensity was determined using ImageJ software (at least 15 nematodes per group).